Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar;32(3):545–557. doi: 10.1101/gr.276139.121

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Li et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see https://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 3. — RNase footprinting examines mitochondrial translation. (A) The distribution of footprint lengths across mitochondrial protein-coding regions. (B) The fractions of reads in the three codon frames. The 32-nt-long footprints (the peak size) in mitochondrial coding regions were used for the calculation. The SD represents the variation across 13 mitochondria-encoded protein-coding genes. (C) An example transcript region of MT-CO1, showing the 3-nt periodicity of read distribution. (D) We used the sucrose gradient to isolate 55S mitoribosome and 80S ribosome complexes and then extracted associated footprints for sequencing. We performed western blots for ribosomal proteins to show the fractions containing 55S mitoribosomes and 80S ribosomes. (E) The correlation of footprint length distribution in mitochondrial coding regions calculated using our RNase footprinting and sucrose gradient-based ribosome profiling. (F,G) The correlation of ribosome occupancies in mitochondrial-encoded (F) and nuclear-encoded (G) coding genes calculated using the two methods. The RNase footprinting data using 50,000 HEK293T cells were used for the analyses.