Table 1.
Structural parameters from SAXS and MALS data
| SAXS sample SASBDB# | Dmax (Å) | Rg (Å) from Guinier plot | Rg (Å) from P(r) | MW Seq. monomer (kDA) | MW SAXS (kDa) | MW MALS (kDa) | Model fit χ2 | data source |
|---|---|---|---|---|---|---|---|---|
| KUΔCTR SASDJV4 | ~ 125 | 37.9±0.6 | 37.9 | 134 | ~ 140 | 145 | 1.7 | SEC-SAXS (This study) |
| KU SASDJU4 | ~ 155 | 41.5±1.4 | 42.7 | 153 | ~ 160 | 160 | 1.7 | SEC-SAXS (This study) merged with SAXS from [27] |
| KU-DNA SASDJW4 | ~ 155 | 40.7±0.3 | 41.9 | 173 | ~ 170 | 190 | 2.6 | Data from [27] |
| DNA-PKcs SASDJX4 | ~ 155 | 57.1±1.7 | 54.4 | 469 | ~ 480 | 480 | 5.9 | SEC-SAXS from [30] merged with SAXS from [27] |
| Autophospho. DNA-PKcs SASDJY4 | ~ 160 | 57.2±1.7 | 56.0 | 469 | ~ 480 | ND | 3.7 | SAXS from [27] |
| DNA-PK monomer SASDJZ4 | ~ 230 | 65.1±0.6 | 66.8 | 640 | ~ 590 | 640 | 3.7 | SEC-SAXS from [30] |
| DNA-PK monomer/dimer SASDJ25 | ~ 300 | 75.4±1.5 | 83.5 | 640 | ~ 720 | 680–700 | 1.0 | SEC-SAXS from [30] |
| DNA-PKcs – 40bp DNA | ~ 315 | 80.9 ± 4.2 | 92.9 | 499 | ~860 | ND | 1.8 | SAXS from [27] |
| DNA-PKcs – 40bp H-DNA | ~ 315 | 87.7 ± 6.3 | 96.4 | 497 | ~900 | ND | 1.3 | SAXS from [27] |
| DNA-PKcs – 40bp Y-DNA | ~ 315 | 90.2 ± 4.1 | 101.2 | 501 | ~880 | ND | 5.0 | SAXS from [27] |