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. 2022 Mar 2;42(9):1666–1678. doi: 10.1523/JNEUROSCI.1466-21.2022

Figure 4.

Figure 4.

mGluR-LTD induces degradation of Synpo in dendritic shafts. A, Representative images and quantification of immunolabeled Synpo in MAP2-positive dendrites of hippocampal neurons treated with vehicle (Ctrl) or DHPG followed by recovery for 30 or 120 min. Scale bars, 15 μm. B, Quantification of dendritic Synpo clusters per cell (fold vehicle) from images as in A. Data are mean ± SE. 30 min, Ctrl 1.0 ± 0.14, DHPG 0.59 ± 0.053, n = 71 cells per group from N = 6 experiments. *p = 0.0106 (120 min). Ctrl 1.0 ± 0.111 (n = 44), DHPG 0.69 ± 0.083 (n = 45), N = 4. **p = 0.004 (paired t test). C, Representative immunoblot of cortical neurons treated with vehicle (Ctrl) or DHPG followed by 30 min recovery: tubulin (Tub), loading control. D, Quantification of Synpo (fold Ctrl) from blots as in C. Data are mean ± SE. Ctrl 1.0 ± 0.14, DHPG 0.69 ± 0.13, N = 5. ***p = 0.0002 (paired t test). E, Representative images of Synpo in hippocampal neurons treated with vehicle, Bay, or DHPG (in the presence/absence of Bay) followed by 30 min recovery. Scale bar, 15 μm. F, Quantification of Synpo clusters from images as in E. Data are mean ± SE. Vehicle 1.0 ± 0.069 (n = 17), DHPG 0.66 ± 0.044 (n = 28), Bay 0.84 ± 0.049 (n = 26), DHPG+Bay 0.95 ± 0.058 (n = 18) from 2 experiments. ***p < 0.001. Not significant, p > 0.1 (one-way ANOVA with Tukey's post-test). G, Representative images of Synpo in hippocampal neurons treated with vehicle, MPEP, or DHPG in the presence/absence of MPEP followed by 30 min recovery. Scale bar, 15 μm. H, Quantification of Synpo clusters from images as in G. Data are mean ± SE. Vehicle 1.0 ± 0.039 (n = 29), DHPG 0.49 ± 0.052 (n = 16), MPEP 0.66 ± 0.038 (n = 32), DHPG+MPEP 0.66 ± 0.035 (n = 31); from 4 experiments. ***p < 0.001 (one-way ANOVA with Tukey's post-test).