Fig. 1.

Visual comparison of vimentin marker densities for each transformation method. Density plots for the median cell intensity of the marker vimentin, where each color represents a different slide in the dataset. Each row is aligned with the scale transformations present in Table 1, where each column also matches with the normalization algorithms in Table 1. The ticks on the x-axis represent the Otsu thresholds for each slide for that transformed data, where the color again corresponds to the slide (such that the colors are one-to-one between threshold and density plot). Anderson–Darling test statistics for the marker vimentin are presented for each method in the top right corner