Figure 2.

CsUGT87E7 catalyzed the glycosylation of SA in vitro and induced by SA treatment and fungal infection in tea plants. A, SA-responsive UGT genes identified in the tea plant transcriptome after SA treatment. Cloned genes are marked by a triangle and CsUGT87E7 characterized in this study is boxed. B, The enzymatic reaction product was detected by LC–MS in negative ion mode, using an authentic standard and the empty vector as controls. C, Mass spectrum of the product formed by CsUGT87E7. D, The kinetics of CsUGT87E7 was measured under optimized conditions of reaction time. E and F, RT-qPCR analysis of CsUGT87E7 relative expression level induced by SA treatment (E) and by fungal infection (F). The GAPDH was used as the internal reference gene. Data are presented as mean ± sd of at least three biological replicates. Significant differences between the treatment and the control group were calculated by one-way ANOVA in SPSS 21.0. *P < 0.05, **P < 0.01.