Table 1.
The antibody sources and concentrations used for Western blotting and immunochemical staining
| Target proteins | Antibody brand (series number) | Concentration for Western blotting | IHC concentration for immunochemical staining |
|---|---|---|---|
| E-cadherin | BD transduction Laboratories (610182) | 1:5,000 | 1:200 |
| CK5 | Abcam (ab52635) | 0.388888889 | 1:100 |
| CK14 | Abcam (ab9220) | 1:1,000 | 1:25 |
| CK20 | Abcam (ab76126) | 1:5,000 | 1:100 |
| Shh | Abcam (ab53281) | 1:10,000 | 1:300 |
| ZO-1 | GeneTex (GTX108627) | 1:1,000 | - |
| CD34 | Abcam (ab81289) | 1:10,000 | - |
| ICAM-1 | Abcam (ab53013) | 1:1,000 | - |
| tryptase | Sigma-Aldrich (MAB1222) | 1:1,000 | - |
| TGF-β | CellSignaling (56E4) | 1:1,000 | - |
| GAPDH | GeneTex (GTX100118) | 1:100,000 | - |
IHC, immunohistochemistry; CK, cytokeratin; Shh, sonic hedgehog; ZO-1, zonula occludens-1; ICAM-1, intercellular adhesive molecule-1; TGF-β, transforming growth factor-β; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.