Table 3.
The Western blot quantification results of changes of urothelial cell proliferation, cytoskeleton, inflammation, and barrier function protein expression after repeat PRP injections
| Biomarker | Patient | Baseline | After PRP | P-value |
|---|---|---|---|---|
| Shh | Total patients | 0.12 ± 0.05 | 0.29 ± 0.20 | 0.002 |
| HIC | 0.06 ± 0.02 | 0.13 ± 0.05 | 0.156 | |
| NHIC | 0.21 ± 0.26 | 0.36 ± 0.25 | 0.004 | |
| CD34 | Total patients | 0.70 ± 0.46 | 0.60 ± 0.33 | 0.467 |
| HIC | 0.27 ± 0.16 | 0.38 ± 0.20 | 0.602 | |
| NHIC | 0.74 ± 0.42 | 0.62 ± 0.32 | 0.391 | |
| CK5 | Total patients | 0.33 ± 0.10 | 0.68 ± 0.34 | 0.027 |
| HIC | 0.26 ± 0.16 | 1.06 ± 0.06 | 0.054 | |
| NHIC | 0.74 ± 1.09 | 0.8 ± 0.55 | 0.135 | |
| CK14 | Total patients | 0.25 ± 0.10 | 0.39 ± 0.36 | 0.136 |
| HIC | 0.18 ± 0.05 | 0.02 ± 0.01 | 0.118 | |
| NHIC | 0.37 ± 0.30 | 0.48 ± 0.37 | 0.080 | |
| NHIC | 0.27 ± 0.10 | 0.44 ± 0.36 | 0.082 | |
| CK20 | Total patients | 0.19 ± 0.19 | 0.10 ± 0.13 | 0.095 |
| HIC | 0.07 ± 0.01 | 0.11 ± 0.05 | 0.453 | |
| NHIC | 0.25 ± 0.27 | 0.17 ± 0.29 | 0.071 | |
| ZO-1 | Total patients | 0.17 ± 0.08 | 0.47 ± 0.28 | 0.002 |
| HIC | 0.33 ± 0.01 | 0.27 ± 0.07 | 0.383 | |
| NHIC | 0.14 ± 0.06 | 0.5 ± 0.29 | 0.001 | |
| E-cadherin | Total patients | 0.46 ± 0.44 | 0.68 ± 0.54 | 0.001 |
| HIC | 0.33 ± 0.12 | 0.31 ± 0.05 | 0.877 | |
| NHIC | 0.48 ± 0.46 | 0.72 ± 0.55 | < 0.001 | |
| TGF-β | Total patients | 0.31 ± 0.46 | 0.80 ± 0.28 | 0.000 |
| HIC | 0.16 ± 0.18 | 0.94 ± 0.39 | 0.072 | |
| NHIC | 0.38 ± 0.46 | 0.75 ± 0.25 | 0.003 | |
| ICAM-1 | Total patients | 2.26 ± 2.63 | 0.74 ± 0.71 | 0.054 |
| HIC | 4.23 ± 0.96 | 1.33 ± 0.39 | 0.204 | |
| NHIC | 1.54 ± 2.24 | 0.76 ± 0.73 | 0.201 | |
| Mast cell activity (tryptase) | Total patients | 1.58 ± 4.14 | 1.19 ± 1.44 | 0.560 |
| HIC | 0.72 ± 0.51 | 0.50 ± 0.55 | 0.820 | |
| NHIC | 1.68 ± 4.38 | 1.27 ± 1.50 | 0.584 |
Values are presented as mean±standard deviation.
The data of Western blot quantification results presented as the ratio of different targets/GAPDH. The results in total patients (n=19), HIC (IC/BPS with Hunner’s lesion, n=2) and NHIC (IC/BPS without Hunner’s lesion, n=17) were presented separately.
GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PRP, platelet-rich plasma; Shh, sonic hedgehog; IC/BPS, interstitial cystitis/bladder pain syndrome; CK, cytokeratin; ZO-1, zonula occludens-1; TGF-β, transforming growth factor-β; ICAM-1, intercellular adhesive molecule-1.