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. 2022 Mar 4;8(9):eabj4641. doi: 10.1126/sciadv.abj4641

Fig. 7. TpMos display protective functions during sepsis by balancing the proinflammatory functions of MatMos.

Fig. 7.

(A) TpMos and MatMos were sorted and analyzed for the expression of IL-1β, TNF-α, and iNOS after stimulation with LPS. (B) Control and CLP-induced septic BM TpMos and MatMos were stimulated with LPS and analyzed for the expression of IL-1β, TNF-α, and iNOS. (C) LPS-stimulated control BM TpMos and MatMos were analyzed for apoptotic cells using FLICA Poly Caspase. (D) TpMos and MatMos were sorted from CD45.1 mice and adoptively transferred into CLP-induced CD45.2 recipient mice via the intraperitoneal route. Analysis of PL of recipient mice for transferred cells was performed on days 1, 3, and 5 after adoptive transfer for the expression of IL-6, IL-1β, TNF-α, and iNOS. Results are expressed as means ± SD (n = 4 to 6) and representative of one of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (Student’s t test). (E) TpMos or MatMos were sorted and adoptively transferred into recipient mice shortly after they were subjected to CLP. The mortality of these recipient mice was assessed using the Kaplan-Meier survival curve. Results are representative of one of three independent experiments (n = 10). *P < 0.05 (Mantel-Cox).