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. 2022 Mar 4;8(9):eabj0112. doi: 10.1126/sciadv.abj0112

Fig. 7. Microglia depletion stabilizes synapse changes after OGD.

Fig. 7.

(A) BDNF expression within microglia in BL6 WT sham and MCAO animals. (A′) Quantification of BDNF intensity within IBA1-positive cells. *P < 0.05. (B) Example images from BL6 WT mice treated with PLX5622 sham and MCAO. (B′) Microglia depletion after PLX5622 chow treatment for 7 days (chow, 1200 mg/kg). ***P < 0.001. (C) Example images from BL6 WT PLX5622-treated MCAO animals, quantified for (D and E) VGLUT1 and PSD95 and (F to H) GAD65/67, GABAAR γ2, and GABAAR α5 expression changes relative to sham in BL6 WT PLX5622-treated MCAO animals. (I) WB analysis for total gephyrin, phospho-gephyrin at Ser268, and phospho-gephyrin at Ser270 levels in BL6 WT mice treated with PLX5622 at 24 hours after MCAO. (I′ to I‴) Quantification of WB for total gephyrin, phospho-gephyrin at Ser268 and phospho-gephyrin at Ser270 levels in BL6 WT mice treated with PLX5622 at 24 hours after MCAO. Quantification expressed as means ± SD (n = 5); P > 0.05 (one-way ANOVA, Bonferroni multiple comparison test).