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. 2022 Mar 4;8(9):eabj0112. doi: 10.1126/sciadv.abj0112

Table 1. Antibody list.

GFAP, glial fibrillary acidic protein.

Target Host species Dilution Catalog no. Company/origin
Antibodies used for immunohistochemistry
GABAAR γ2 Guinea pig 1:2000 Homemade
GABAAR α5 Guinea pig 1:3000 Homemade
GAD65/67 Rabbit 1:1000 GC 3008 Affiniti
VGLUT1 Guinea pig 1:1000 135 304 Synaptic Systems
PSD95 Mouse 1:1000 73-028 NeuroMap
GFAP Mouse 1:2000 MAB360 Millipore
IBA1 Rabbit 1:1000 019-19741 Wako
CD11b Rat 1:50 550282 BD Pharmingen
mBDNF N-9 Mouse 1:100 BDNF #9 DSHB
p75NTR Rabbit 1:200 AB-NO1 Advanced Targeting System
proBDNF #B240 Mouse 1:200 Philip Barker
Antibodies used for WB
GABAAR α1 Rabbit 1:600 Homemade
GABAAR α2 Rabbit 1:1000 Homemade
Gephyrin Mouse 1:1000 147 111 Synaptic Systems
Gephyrin pS268 Rabbit 1:500 Homemade
Gephyrin pS270 Rabbit 1:500 Homemade
GSK3β Rabbit 1:1000 ab124661 Abcam
ERK1/2 Rabbit 1:4000 9102S Cell Signaling Technology
Phospho-Erk1/2 (Thr202/Tyr204) Rabbit 1:4000 9101 Cell Signaling Technology
BDNF Rabbit 1:3000 Ab108319 Abcam
BDNF Mouse 1:3000 Ab327 Icosagen
Actin Mouse 1:10,000 MAB1501 Millipore