Table 1. Antibody list.
Target | Host species | Dilution | Catalog no. | Company/origin |
Antibodies used for immunohistochemistry | ||||
GABAAR γ2 | Guinea pig | 1:2000 | − | Homemade |
GABAAR α5 | Guinea pig | 1:3000 | − | Homemade |
GAD65/67 | Rabbit | 1:1000 | GC 3008 | Affiniti |
VGLUT1 | Guinea pig | 1:1000 | 135 304 | Synaptic Systems |
PSD95 | Mouse | 1:1000 | 73-028 | NeuroMap |
GFAP | Mouse | 1:2000 | MAB360 | Millipore |
IBA1 | Rabbit | 1:1000 | 019-19741 | Wako |
CD11b | Rat | 1:50 | 550282 | BD Pharmingen |
mBDNF N-9 | Mouse | 1:100 | BDNF #9 | DSHB |
p75NTR | Rabbit | 1:200 | AB-NO1 | Advanced Targeting System |
proBDNF #B240 | Mouse | 1:200 | Philip Barker | |
Antibodies used for WB | ||||
GABAAR α1 | Rabbit | 1:600 | − | Homemade |
GABAAR α2 | Rabbit | 1:1000 | − | Homemade |
Gephyrin | Mouse | 1:1000 | 147 111 | Synaptic Systems |
Gephyrin pS268 | Rabbit | 1:500 | − | Homemade |
Gephyrin pS270 | Rabbit | 1:500 | − | Homemade |
GSK3β | Rabbit | 1:1000 | ab124661 | Abcam |
ERK1/2 | Rabbit | 1:4000 | 9102S | Cell Signaling Technology |
Phospho-Erk1/2 (Thr202/Tyr204) | Rabbit | 1:4000 | 9101 | Cell Signaling Technology |
BDNF | Rabbit | 1:3000 | Ab108319 | Abcam |
BDNF | Mouse | 1:3000 | Ab327 | Icosagen |
Actin | Mouse | 1:10,000 | MAB1501 | Millipore |