| Genetic reagent (Mus musculus, male) |
NLRP3-deficient mice (Nlrp3-/-) - C57 BL/6 Nlrp3tm1Vmd
|
Jackson laboratories |
RRID:MGI:5468973
|
In vivo mouse models and biological samples. |
| Genetic reagent (Mus musculus, male) |
ASC-deficient mice (Pycard-/-) - C57 BL/6 Pycardtm1Vmd
|
Jackson laboratories |
RRID:MGI:3047277
|
In vivo mouse models and biological samples. |
| Genetic reagent (Mus musculus, male) |
Casp1/11-deficient mice (Casp1/11-/-) - B6N.129S2-Casp1tm1Flv
|
Jackson laboratories |
RRID:MGI:5467384
|
Biological samples. |
| Antibody |
Anti-GSDMD (rabbit monoclonal, clone EPR19828) |
Abcam |
Cat#: ab209845, RRID:AB_2783550
|
WB (1:2000) |
| Antibody |
Anti-Caspase 1 p20 (mose monoclonal, clone Casper-1) |
Adipogen |
casper-1, Cat# AG-20B-0042, RRID:AB_2490248
|
WB (1:1000) |
| Antibody |
Anti-IL-1β (rabbit polyclonal) |
Santa Cruz |
H-153Cat#: SC-7884, RRID:AB_2124476
|
WB (1:1000) |
| Sequence-based reagent |
KiCqStart SYBR Green Primers |
Sigma-Aldrich |
Tnfa(NM_013693)Il6(NM_031168)Il1b(NM_008361)Actb(NM_007393)Cox2 (Ptgs2)(NM_011198)Arg1(NM_007482)Fizz1 (Retnlb)(NM_023881)Mrc1(NM_ 008625)Ym1 (Chil1)(NM_007695001080219) |
qRT-PCR |
| Commercial assay or kit |
ELISA for IL-1β |
R&D SystemsThermo Fisher Scientific |
Cat# 88-7013-88, RRID:AB_257494688-7013-88xxx
|
ELISA for cell supernatant |
| Commercial assay or kit |
ELISA for TNFα |
Thermo Fisher Scientific |
Cat# 88-7324-88, RRID:AB_257508088-7324-88xxx
|
ELISA for cell supernatant |
| Commercial assay or kit |
ELISA for IL-6 |
R&D Systems |
Cat# M6000B, RRID:AB_2877063 M6000Bxxx |
ELISA for cell supernatant |
| Commercial assay or kit |
ELISA for IL-18 |
Thermo Fisher Scientific |
Cat# BMS618/3, RRID:AB_2575692BMS618-3xxx
|
ELISA for cell supernatant |
| Chemical compound, drug |
MCC950 |
Sigma-Aldrich |
PZ0280 |
Cell culture:10 µM |
