Figure 4. Simultaneous detection of 5-hydroxymethylcytosine (5hmC) and R-loops at the same genomic loci in individual mouse embryonic stem (mES) cells.

(A) 5hmC and R-loops proximity ligation assay (PLA) foci in mES cells. DAPI was added to the mounting medium to stain DNA. Scale bars: 10 μm. Data are representative of at least three independent experiments with similar results. (B) Boxplot showing 5hmC/R-loops PLA foci per nucleus. Horizontal solid lines represent the median values, and whiskers correspond to the 10 and 90 percentiles. A minimum of 300 cells from at least three independent experiments was scored for each experimental condition. ****p<0.0001, Mann–Whitney rank test.