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. 2022 Mar 5;70:103219. doi: 10.1016/j.jddst.2022.103219

Table 3.

Recent relevant research of micelles for respiratory therapy.

Materials Disease Drug Fabrication method Efficacy tests Cytotoxicity tests Reference
Chitosan Tuberculosis Rifampicin/pyrazinamide Dialysis In vitro MIC test MTT assay, negligible cytotoxicity (VERO cells), high toxicity (TPH-1 cells) [6]
Inulin-vitamin E Tuberculosis Rifampicin Dialysis In vitro MIC test MTT assay (human alveolar macrophages), viability 63% (2 mg/mL) [37]
Chitosan-graft-poly(caprolactone)/(ferulic acid) Tuberculosis Rifampicin Dialysis In vitro inhibition test Hemocytometer with trypan blue exclusion (A549 cells), IC50 of ∼60 μg/mL* [38]
α-tocopheryl succinate-PEG Lung cancer PTX Thin-film hydration In vitro MTT assay MTT assay (A549 cells), IC50 ∼1.5 μg/mL* [39]
Poly (vinyl caprolactam)-poly (vinyl acetate)-PEG Tuberculosis Rifampicin Solvent-diffusion In vitro MIC test CellTiter 96® (RAW 264.7), cell viability >85% (100 μg/mL) [40]
Pluronic® 123/Pluronic® F127 Lung inflammation Budesonide Thin-film hydration In vivo inflammation model (male Wistar rat) Non reported [41]
PEG-PLA/Pluronic® P105 Lung cancer PTX Thin-film hydration In vivo tumor growth inhibition (BALB/c nude mice) MTT assay (A549 cells), IC50 ∼87.09 ng/mL [42]
Soluplus®/d-α-tocopherol acid polyethylene glycol 1000 succinate)/dequalinium Lung cancer Hinokiflavone Thin-film hydration In vivo tumor growth inhibition (BALB/c nude mice) Cell counting kit-8 (A549 cells), IC50 7.81 μg/mL [43]
Cholesterol-conjugated PAMAM Acute lung injury Resveratrol/heme oxygenase-1 gene Emulsification-solvent evaporation In vivo lipopolysaccharide induce acute lung injury model (BALB/c nude mice) MTT assay (L2 cells), cell viability >80% (15 μg/mL) [44]

Abbreviations in table: MIC = minimum inhibitory concentration, IC50 = half maximal inhibitory concentration, MTT = 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, VERO = african green monkey kidney epithelial cells, TPH-1 = human leukemia monocytic cells, A549 = adenocarcinomic human alveolar basal epithelial cells, RAW 264.7 = mouse leukemic monocyte-macrophage cells, * = value read from a graph.