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. 2022 Feb 25;2022:6324292. doi: 10.1155/2022/6324292

Figure 2.

Figure 2

ROS scavenger prevents the GLB-induced DNA damage and proliferation inhibition. (a, b) A2780 or A2780/DDP cells were pretreated with ROS scavenger NAC (5 mM) for 1 h and then treated with GLB for 2 h; the intracellular ROS levels were monitored by fluorescent probe DCFH-DA. Scale bar = 100 μm. (c) A2780 or A2780/DDP cells were pretreated with NAC for 1 h and then treated with GLB for 20 h; the formation of 53BP1 foci was analyzed using a fluorescence microscope. Scale bar = 5 μm. (d, e) A2780 or A2780/DDP cells were pretreated with NAC for 1 h; the colony formation ability was measured by colony-forming assay after treatment with GLB. (f) A2780 or A2780/DDP cells were pretreated with NAC for 1 h and then treated with GLB for 24 h; the cell viability was determined by trypan blue exclusion. Data from three technical replicates (∗∗∗p < 0.001).