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. 2022 Feb 25;2022:6324292. doi: 10.1155/2022/6324292

Figure 3.

Figure 3

JNK signaling pathway is involved in GLB-induced cell death. (a, b) A2780 or A2780/DDP cells were treated with GLB at different time points. The protein levels of p-JNK were detected by western blot. (c–e) A2780 or A2780/DDP cells were treated with GLB at different concentrations. The protein levels of p-JNK were detected by western blot. (f, g) A2780 or A2780/DDP cells were pretreated with JNK inhibitor SP600125 (20 μM) for 1 h before exposing to GLB; the protein levels of p-JNK were detected by western blot. (h) A2780 or A2780/DDP cells were pretreated with JNK inhibitor SP600125 (20 μM) for 1 h before exposing to GLB; the cell viability was determined by trypan blue exclusion. (i–k) A2780 or A2780/DDP cells were pretreated with NAC for 1 h before exposing to GLB; the protein levels of p-JNK were detected by western blot. Data from three technical replicates (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001).