Figure 1.

AAV8-mediated transduction of VMN cells with PAC1R shRNA decreases PAC1R protein expression. A: ×10 stitched confocal photomicrograph of VMN cells 21 days after transduction with AAV8 containing control or PAC1R-shRNA (scale bar = 100 µm). Inset is a ×20 magnification of the injection area (scale bar = 50 µm). B: representative Western blots and quantification of PAC1R protein expression in VMN tissue from Sprague-Dawley rats injected with AAV8 nonsilencing control (NSc) or PAC1R shRNA. Data are expressed as means ± SE, *P < 0.05 compared with control (n = 8 rats/group). ARC = arcuate nucleus, 3V = third ventricle; PAC1R, pituitary adenylate cyclase receptor; VMN, ventromedial nuclei.