Figure 2.

Mitochondrial peripheral blood mononuclear cell (PBMC) function in high-fit and low-fit females at baseline and after a recent bout of exercise. Representation of mitochondrial parameters derived from the extracellular flux (XF) assay using injection strategy A (A) or B (B) and traces for low-fit (n = 16, white) and high-fit (n = 15, black) at baseline (dots) and postexercise (triangles). Basal oxygen consumption rate (OCR) (C), FCCP-induced OCR (D), and corresponding spare respiratory capacity (E). ATP-linked OCR (F), proton leak (G), and corresponding coupling efficiency (H). Parameters are calculated for low-fit (n = 16, gray) and high-fit (n = 15, blue) females at baseline (clear bars) or postexercise (striped bars). Values are depicted per 10⁵ R-integrated pixel intensity (PIXI) analyzed cells for absolute rates or as a percentage (%) for ratios of corresponding values. Main effects (fitness level and recent exercise) and interaction effects were analyzed using repeated-measures ANOVA (RM-ANOVA). Significant P values (<0.05) are indicated in bold.