Table 2.
Female characteristics
| Female | WT NC, n = 12 | WT HFD, n = 12 | KO NC, n = 11 | KO HFD, n = 9 | Main Effect |
|---|---|---|---|---|---|
| Body weight, g | 21.44 ± 1.37 | 23.73 ± 3.53* | 21.60 ± 1.40 | 24.82 ± 5.32* | Diet (P = 0.006) |
| Soleus, mg | 10.57 ± 1.30 | 10.64 ± 0.91 | 9.50 ± 1.55 | 10.43 ± 2.60 | N/A |
| Gastrocnemius, mg | 106.88 ± 4.74 | 112.82 ± 7.72 | 108.82 ± 11.02 | 112.87 ± 11.28 | N/A |
| TA, mg | 42.10 ± 3.50 | 42.27 ± 4.56 | 41.23 ± 4.04 | 38.48 ± 2.86 | Geno (P = 0.06) |
| Plantaris, mg | 15.85 ± 1.64 | 17.05 ± 1.45 | 15.14 ± 1.57* | 15.26 ± 1.34* | Geno (P = 0.013) |
| EDL, mg | 9.35 ± 0.74 | 9.56 ± 1.13 | 9.21 ± 0.89 | 9.74 ± 1.27 | N/A |
| Heart, mg | 112.53 ± 12.00 | 127.63 ± 15.57* | 112.34 ± 7.29 | 125.92 ± 12.82* | Diet (P = 0.001) |
| Liver, mg | 1,001.07 ± 71.93ab | 878.14 ± 107.19a | 910.36 ± 131.36ab | 1,009.52 ± 228.70b | Interaction (P = 0.013) |
| Lungs, mg | 152.38 ± 17.15 | 151.83 ± 23.26 | 153.90 ± 17.60 | 165.72 ± 13.47 | N/A |
| Spleen, mg | 100.91 ± 12.00a | 93.39 ± 13.87ab | 82.93 ± 9.43b | 98.73 ± 16.01a | Interaction (P < 0.006) |
| Fat, mg | 167.43 ± 78.15 | 443.30 ± 244.46* | 226.06 ± 97.87 | 481.73 ± 302.75* | Diet (P < 0.001) |
| Tibia length, mm | 17.04 ± 0.1 | 17.10 ± 0.09 | 17.03 ± 0.09 | 17.10 ± 0.08 | N/A |
Tissue weights are recorded as wet weights. All values are represented as means ± SE. An n of 10–12 animals per group was used. EDL, extensor digitorum longus; HFD, high-fat diet; KO, knockout; NC, normal chow; TA, tibialis anterior; WT, wild type. Superscript “different letters” denote an interaction effect with α value set at P < 0.05.
Data analyzed by two-way ANOVA with Tukey’s post hoc test where appropriate. *: Statistical significance for main effect of genotype or diet with α value set at P < 0.05.