Figure 5.

qPCR analysis of the BSH-expressing L. acidophilus and L. johnsonii as well as the DNA encoding BSH. The primer sequences were described as we reported before^30,56 and in Table S2. All primers were synthesized by Integrated DNA Technologies. The abundance of the genomic DNA encoding the bacterial 16S rRNAs in the intestinal content of mice was determined by quantitative polymerase chain reaction (qPCR) using a CFX384 Real-Time PCR Detection System. Results are expressed as the mean (SE) delta–delta cycle value (calculated as 2ˆ[−(Cq − average reference Cq)]) of the quantitative PCR as compared with the universal bacteria, per nanogram of DNA from the intestinal content. (a) and (b) represent statistically significant differences compared to post hoc groups. Treatment groups that are not statistically different are labeled with the same letter.