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Immunohistochemistry. A-D) immunohistochemistry of collagen type I, OPN, TRAP, and CD31. E-H) quantitative results in the proportion of positive areas in collagen I, OPN, TRAP, and CD31 (n = 3, all values were mean ± std. dev., NS, not significant, *p < 0.05, **p < 0.01, ***p < 0.001 when comparing A-H + AB-O and other groups via two-way ANOVA analysis followed by Tukey's multiple comparison test by GraphPad Prism 7.0 Software (USA)).
