Fig. 1.

BMSCs promote the migration and tubule formation of RAECs by regulating cell adhesion. (A) Schematic diagram of Transwell co-culture system of BMSCs and RAECs. (B)The wound healing rate of RAECs and Co-RAECs in 24h. The distance between the yellow dotted lines represents the scratch gap. (C) Transwell assay was used to detect the migration ability of RAECs and Co-RAECs. (D) Tubule formation ability of RAECs and Co-RAECs, and compared the total branching points between the two groups; (E) Principal component analysis (PCA) of the gene changes. (F) Gene function classification analysis diagram of differential genes. Light-colored columns represent differentially expressed genes, and dark-colored columns represent all genes (including differential and non-differential genes). The light-colored column is higher than the dark-colored column, indicating that the significantly differentially expressed genes have an enrichment trend in this term. (G) Differential gene volcano plot of RAECs and Co-RAECs, the red genes was up-regulated relative to RAECs and green means down-regulated genes. (H) The morphology of RAECs and Co-RAECs was observed by scanning electron microscope (SEM). (I) Cell adhesion rate of RAECs and Co-RAECs. Data were represented as the mean ± SD. **p < 0.01.