Fig. 2.

Nidogen1-enriched EV (EV-NID1) derived from BMSCs may be a key candidate mediator. (A) Schematic diagram of the cytology experiment. RAECs were incubated with concentrated conditioned medium (CM) for 48 h, the EVs were analyzed by TMT proteomics. (B) Wound healing of RAECs incubated with CM or EVs-free-CM (PBS as the Control). (C) Relative migration capacity. (D) Comparison of tubule formation ability and total branching points. (E) The adhesion rate of RAECs in each group. (F) The expression of specific markers (CD63, TSG101 and HSP90) in BMSC-EVs, RAEC-EVs and Co-culture-EVs. (G) Nanoparticle tracking analysis (NTA) showed the particle size distribution of BMSC-EVs. (H) Transmission electron microscopy (TEM) imaging of BMSC-EVs, Scale bar, 50 nm. (I) Heat map of TMT proteomics analysis. The green box indicates that NID1 enriched in BMSC-EVs and Co-culture-EVs, but absented in RAEC-EVs, and was proposed as a candidate effector molecule. (J) The abundance of NID1 in EVs was verified by Western blot. Data were represented as the mean ± SD. *p < 0.05; **p < 0.01; ns, not significant from Student's t-test.