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. Author manuscript; available in PMC: 2023 Mar 2.
Published in final edited form as: Neuron. 2022 Jan 3;110(5):841–856.e6. doi: 10.1016/j.neuron.2021.12.002

Figure 5.

Figure 5.

VMHvl α cells are highly active in virgin females during mating behaviors.

(A) Schematic illustrating fiber photometry recording in VMHvl and behavioral test in virgin females.

(B-C) PETH of GCaMP6m fluorescence in α cells aligned to onset of (B) male mounting and (C) lordosis.

(F-G) PETH in β aligned to onset of (F) male mounting and (G) lordosis.

(D, H) Representative normalized calcium traces from (D) α cells and (H) β cells during mating interaction. Colored shading marks behavioral episodes.

(E, I) Peak of PETH from (E) α cells and (I) β cells aligned to onset of appetitive phase (sniff) or consummatory phase (male mounting or intromission) during mating interaction.

*p <0.05; ns, not significant. Mean ± SEM.