| Antibodies |
| Rabbit anti-somatostatin-14 (1:250) |
Peninsula Laboratories International |
RRID AB_518614 |
| Goat anti-choline acetyltransferase (1:100) |
Sigma-Aldrich |
RRID AB_2079751 |
| Rabbit anti-mu opioid receptor (1:250) |
ImmunoStar |
RRID AB_572251 |
| Cy™3 AffiniPure Donkey Anti-Rabbit IgG (H+L) (1:500) |
Jackson ImmunoResearch Laboratories |
RRID AB_2307443 |
| Alexa Fluor® 647 AffiniPure Donkey Anti-Goat IgG (H+L) (1:500) |
Jackson ImmunoResearch Laboratories |
RRID AB_2340436 |
| Alexa Fluor® 647 AffiniPure Donkey Anti-Rabbit IgG (H+L) (1:300) |
Jackson ImmunoResearch Laboratories |
RRID AB_2492288 |
| Bacterial and virus strains |
| AAV1-syn-FLEX-jGCaMP7s-WPRE |
Dana et al., 2019 |
Addgene# 104491 |
| AAV1-Syn-GCaMP6f-WPRE-SV40 |
Chen et al., 2013 |
Addgene# 100837 |
| AAV2-EF1a-DIO-eYFP |
Karl Deisseroth |
Addgene# 27056 |
| AAVDJ-EF1a-DIO-hM3D(Gq)-mCherry |
Bryan Roth, Salk Institute Viral Vector Core |
Addgene# 50460 |
| AAVDJ-EF1a-DIO-hM4D(Gi)-mCherry |
Bryan Roth, Salk Institute Viral Vector Core |
Addgene# 50461 |
| AAVDJ-EF1a-DIO-hChR2(H134R)-EYFP-WPRE-pA |
Karl Deisseroth, Salk Institute Viral Vector Core |
Addgene# 20298 |
| AAVDJ-FLEX-ArchT-GFP |
Han et al., 2011, Salk Institute Viral Vector Core |
Addgene# 28307 |
| AAV8-hSyn-FLEX-TVA-P2A-GFP-2A-oG |
John Naughton, Salk Institute Viral Vector Core |
Addgene# 85225 |
| EnvA G-Deleted Rabies-mCherry |
Osakada et al., 2011, Salk Institute Viral Vector Core |
Addgene# 32636 |
| AAV2-phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE |
Oh et al., 2014, Salk Institute Viral Vector Core |
Addgene# 51509 |
| AAVrg-EF1a-DIO-FLPo-WPRE-hGHpA |
Zingg et al., 2017, Salk Institute Viral Vector Core |
Addgene# 87306 |
| AAVrg-EF1a-DIO-mCherry |
Bryan Roth, Salk Institute Viral Vector Core |
Addgene# 50462 |
| AAV8-Ef1a-fDIO-GCaMP6s |
Rylan Larsen, Salk Institute Viral Vector Core |
Addgene# 105714 |
| AAV9-EF1a-fDIO-hChR2(H134R)-EYFP |
Fenno et al., 2014, Salk Institute Viral Vector Core |
Addgene# 55639 |
| AAV8-EF1a-fDIO-EYFP |
Fenno et al., 2014, Salk Institute Viral Vector Core |
Addgene# 55641 |
| AAVDJ-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA |
Runegaard et al., 2018
|
Addgene# 154867 |
| AAV8-hSyn-DO-GCaMP6m |
UNC Viral Vector Core |
N/A |
| AAVDJ-syn-FLEX-jGCaMP8m-WPRE |
Zhang et al., 2020, Janelia Viral Vector Core |
Addgene# 162378 |
| Chemicals, peptides, and recombinant proteins |
| Clozapine N-oxide |
Cayman Chemical |
Cat#16882 |
| Clozapine |
Cayman Chemical |
Cat#12059 |
| HPBCD [(2-Hydroxypropyl)-β-cyclodextrin] |
Alfa Aesar |
Cat#H31133
|
| Cholera Toxin Subunit B-488 |
Invitrogen |
Cat#C22841
|
| CNQX |
Tocris |
Cat#0190 |
| RNAscope Probe: Oprm1
|
Advanced Cell Diagnostics |
Cat#315841 |
| RNAscope Probe: Calca
|
Advanced Cell Diagnostics |
Cat#578771 |
| RNAscope Probe: Tac1
|
Advanced Cell Diagnostics |
Cat#410351 |
| RNAscope Probe: Htr2a
|
Advanced Cell Diagnostics |
Cat#401291 |
| RNAscope Probe: Pdyn
|
Advanced Cell Diagnostics |
Cat#318771 |
| RNAscope Probe: FoxP2
|
Advanced Cell Diagnostics |
Cat#428791 |
| RNAscope Probe: Tacr1
|
Advanced Cell Diagnostics |
Cat#428781 |
| Deposited data |
| Custom MATLAB scripts for data analysis |
This paper |
https://doi.org/10.5281/zenodo.5685145
|
| Experimental models: Organisms/strains |
| Mouse: Oprm1-Cre:GFP |
Laboratory of Dr. Richard Palmiter |
N/A |
| Mouse: wild type C57BL/6J |
Jackson Laboratory |
Stock No. 000664 |
| Software and algorithms |
| MATLAB |
MathWorks |
R2019a |
| Python |
Anaconda Distribution |
Version 3.7 |
| Doric Neuroscience Studio |
Doric Lenses |
N/A |
| RStudio |
RStudio |
Version 1.2.5001 |
| rEDM |
https://cran.r-project.org/web/packages/rEDM/
|
Version 0.7.2 |
| rEDM |
https://ha0ye.github.io/rEDM/
|
Version 0.7.4 |
| LabChart |
ADInstruments |
Version 8 |
| EthoVision XT |
Noldus Information Technology |
Version 12 |
| BZ-X viewer |
Keyence |
N/A |
| OlyVIA |
Olympus Life Science |
N/A |
| pClamp |
Molecular Devices |
Version 10 |
| PRISM |
GraphPad Software |
Version 6 |
| Illustrator |
Adobe |
Version CS6 and CC2018 |
| Inscopix Data Processing Software |
Inscopix |
Version 1.6.0.3225 |
| Other |
| 200um 0.22 NA Fiber-optic Cannula |
Laboratory of Dr. Sung Han |
N/A |
| 400um 0.37 NA Fiber-optic Cannula |
Laboratory of Dr. Sung Han |
N/A |
| ProView™ Integrated Lens 0.6mm x 7.3mm |
Inscopix |
Cat# 1050–004413 |
