Table 1. List of primer pairs for real-time quantitative PCR.
Reference genes: Actβ, Gapdh, Hprt. Actβ = β-actin; Agrp = agouti-related peptide; Cart = cocaine- and amphetamine-regulated transcript; Npy = neuropeptide Y; Pomc = proopiomelanocortin; Gapdh = glyceraldehyde-3-phosphate dehydrogenase; Ghsr = growth hormone secretagogue receptor (ghrelin receptor); Hprt = hypoxanthine-guanine phosphoribosyltransferase; Insr = insulin receptor; Lepr = leptin receptor; Esr1 = estrogen receptor 1 (ERα).
| Gene Name |
Product Length |
Primer Eff. (%) |
Primer sequence | Base Pair # | Accession # |
|---|---|---|---|---|---|
| Actβ | 63 | 101 | F:GCCCTGAGGCTCTTTTCCA R:TAGTTTCATGGATGCCACAGGA |
849-867 890-911 |
NM_007393.3 |
| Agrp | 146 | 105 | F:CTCCACTGAAGGGCATCAGAA R:ATCTAGCACCTCCGCCAAA |
287-307 414-432 |
NM_007427.2 |
| Cart | 169 | 95 | F:GCTCAAGAGTAAACGCATTCC R:GTCCCTTCACAAGCACTTCAA |
227-297 425-445 |
NM_013732 |
| Gapdh | 98 | 93 | F:TGACGTGCCGCCTGGAGAAA R:AGTGTAGCCCAAGATGCCCTTCAG |
778-797 852-875 |
NM_008084.2 |
| Ghsr | 122 | 123 | F:CAGGGACCAGAACCACAAAC R:AGCCAGGCTCGAAAGACT |
1003-1022 1107-1124 |
NM_177330 |
| Hprt | 117 | 107 | F:GCTTGCTGGTGAAAAGGACCTCTCGAAG R:CCCTGAAGTACTCATTATAGTCAAGGGCAT |
631-658 718-747 |
NM_013556 |
| Insr | 89 | 114 | F: GTGTTCGGAACCTGATGAC R: GTGATACCAGAGCATAGGAG |
1215-1233 1686-1706 |
NM_010568 |
| Lepr | 149 | 105 | F:AGAATGACGCAGGGCTGTAT R:TCCTTGTGCCCAGGAACAAT |
3056-3075 3185-3204 |
NM_146146.2 |
| Npy | 182 | 100 | F:ACTGACCCTCGCTCTATCTC R:TCTCAGGGCTGGATCTCTTG |
106-125 268-287 |
NM_023456 |
| Pomc | 200 | 103 | F:GGAAGATGCCGAGATTCTGC R:TCCGTTGCCAGGAAACAC |
145-164 327-344 |
NM_008895 |
| Esr1 | 113 | 115 | F:AATGTCCACCCGCTAGGCATTC R:CTCCATGTCTTGCGTAGGTCTC |
298-319 389-410 |
NM_010157 |