KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
rAAV1.Syn.Flex.GCaMP6f.WPRE.SV40	This paper	Addgene 100833-AAV1
rAAV8.Ef1α.DIO.ChRmine.oScarlet.WPRE	Karl Deisseroth (Marshel et al., 2019)	N/A
rAAV2/1.Ef1α.DIO.ChRmine.oScarlet.WPRE	This paper	N/A
rAAV8.nEF.Con/Foff.2.0.ChRmine-oScarlet	Karl Deisseroth Fenno et al., 2020	N/A
rAAV1.Syn.GCaMP6f.WPRE.SV40	This paper	Addgene 100837-AAV1
rAAV2/9.Ef1α.FLEX.Breaches.tdTomato	Kaufman et al., 2020	N/A
rAAV2/1.CaMKII.Cre.ERT2	This paper	N/A
rAAV2/1.h56d.HA-hMD4(Gi).mCherry	This paper	N/A
rAAV2/1.h56d.FlpO	This paper	N/A
rAAV8.nEF.Coff/Fon.Arch3.3-eYFP	Karl Deisseroth (Fenno et al., 2020)	N/A
rAAV2/1.Syn.FRT.tdTomato	This paper	N/A
Experimental Models: Organisms/Strains
Mouse: Slc32a1-2A-FlpO-D knock-in (VGAT-FlpO): B6.Cg-Slc32a1tm1.1(flpo)Hze/J	Daigle et al., 2018	Jackson Laboratory Strain 029591
Mouse: R4Ag11: B6.Cg-Tg(CamKIIα-Cre)3Szi/J	Bina Santoro (Dragatsis and Zeitlin, 2000)	Jackson Laboratory Strain 027400
Mouse: R1Ag5: B6.Cg-Tg(CamKIIα-Cre)2Szi/J	Bina Santoro (Dragatsis and Zeitlin, 2000)	Jackson Laboratory Strain 027310
Recombinant DNA
pCAGGS-GCaMP7b	This paper	N/A
pCAGGS-ChRmine-mScarlet	This paper	N/A
Software and Algorithms
Suite2p	Pachitariu et al., 2017	https://github.com/cortex-lab/Suite2P
OASIS	Friedrich et al., 2017	https://github.com/j-friedrich/OASIS
Custom data processing and analysis code	This paper	https://dx.doi.org/10.5281/zenodo.5765716
Python 2.7	https://www.python.org	N/A
SIMA	Kaifosh et al., 2014	https://github.com/losonczylab/sima