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. Author manuscript; available in PMC: 2023 Mar 3.
Published in final edited form as: Mol Cell. 2022 Feb 18;82(5):1003–1020.e15. doi: 10.1016/j.molcel.2022.01.015

Figure 6: EBF1, but not TCF1 or LEF1 alone, maintains chromatin opening.

Figure 6:

(A, B) Differential opening post deletion of LEF1 or TCF1 (A), or both (B) in Cas9-expressing DND41 (DND41-Cas9) at the noted dates. (B, left): the most enriched motifs.

(C) EBF1 levels post its deletion in Cas9-expressing resistant DND41 (DND41-Res-Cas9). D: day; control: Calnexin.

(D, E) Effect of EBF1 loss on apoptosis, death (D) and relative growth (E) in DND41-Res-Cas9 cells. 3 biological with mean ± SD of 5 technical replicates (t-test).

(F) Differential opening and enriched motifs post EBF1 deletion in DND41-Res-Cas9.

(G, H) Differential ATAC-seq post EBF1 deletion in DND41-Res-Cas9 per differential opening (G) or EBF1 binding (H) during resistance development (Wilcoxon test).