Figure 5. Reconstruction and modeling of Lig-PCNA with ligatable DNA after DNA end-joining.
(A) The 4.20 Å cryo-EM map and associated model (B) from the second subset of the cryo-EM dataset of Lig and PCNA with ligatable DNA, in which the DNA has been end-joined. The map and model are viewed from two opposite sides of the complex. The Lig OBD was docked as a rigid body into the incomplete density above PCNA1. The colors are the same as in Figures 3 and 4, except Lig is colored green. See also Figures S3, S4 and S5.