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. Author manuscript; available in PMC: 2023 Mar 15.
Published in final edited form as: J Mol Biol. 2021 Dec 20;434(5):167413. doi: 10.1016/j.jmb.2021.167413

Figure 1. Cryo-EM structure determination of A2Moo.

Figure 1.

(A) Cryo-EM maps of the native-form A2M family protein tetramer (left), locally refined native-form A2M family protein protomer (center), and intermediate-form A2M family protein tetramer (right). (B) Identification of A2Moo, the A2M family protein in Xenopus egg. Top panel; amino acid sequence alignment of sixteen Xenopus laevis A2M family proteins with reasonable protein length to satisfy the EM map. The representative region used for protein identification is shown. A yellow rectangle indicates the protein that matches to the EM density (LOC431886: named A2Moo). Bottom panels; overlay of the atomic model of A2Moo and cryo-EM density of the locally refined native-form A2M protein protomer. Three other representative regions used for protein identification are shown in Figure S5.