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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Respir Physiol Neurobiol. 2022 Feb 3;299:103855. doi: 10.1016/j.resp.2022.103855

Figure 2:

Figure 2:

Locations of the caudal end of the Caudal Medullary Raphe (lower), the preBötzinger Complex (preBötC, middle), and the caudal end of the facial nucleus (7N, upper) as identified by injection of fluorescent latex microspheres in four rabbits. The left panel shows diagrammatic hemisections of the brainstem at each rostro-caudal level with the individual injection sites superimposed in red squares. The right panels show the corresponding hemisections from one animal with immunoreactivity for 5-hydroxytryptophan (5HT), representing serotonergic neurons and choline acetyltransferase (ChAT), representing cholinergic (motor) neurons, superimposed over nuclear staining with DAPI. Red microspheres mark the injection sites. Injection protocols did not differentiate between raphe pallidus (RPa) and raphe obscurus (ROb). IO: inferior olive; py: pyramidal tract; 12N: nucleus hypoglossus; 10N: nucleus nervi vagi; NA: nucleus ambiguus; RMg: raphe magnus. Bar = 1mm. The inserts show zoomed images of the injection sites. Bar = 0.25mm.