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. 2022 Mar 4;13(3):206. doi: 10.1038/s41419-022-04632-z

Fig. 1. Zeb1 is a key regulator of glycolytic gene expression.

Fig. 1

A GSEA of transcriptome data from PyMT;Zeb1cKO vs PyMT cells revealing enrichment of a gene signature associated with reduced glycolysis in PyMT;Zeb1cKO breast cancer cells. NES, normalized enrichment score; FDR, false discovery rate. B Differential expression of glycolytic genes identified by RNA-sequencing. C, D The relative (C) mRNA and (D) protein levels of Zeb1, HK2, PFKP, and PKM2 in PyMT and PyMT;Zeb1cKO cells (n = 5). E The enzyme activities of HK, PFK, and PKM in PyMT and PyMT;Zeb1cKO cells (n = 5). F, G The relative (F) mRNA and (G) protein levels of HK2, PFKP, and PKM2 in empty vector-expressing (Ctrl/231) and Zeb1-expressing (Zeb1/231) MDA-MB-231 cells. H The enzyme activities of HK, PFK, and PKM in Ctrl/231 and Zeb1/231 cells. Dots depict individual samples in (C–E). Data are representative of five (C–E) or three (F–H) independent experiments and presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs the respective control by an unpaired Student’s t-test.