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. 2022 Mar 4;13:1185. doi: 10.1038/s41467-022-28587-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A, B’ XY and YZ views (as indicated) of wing discs expressing mCherryCAAX and either Ths:GFP under ths-Gal4 (A, A”) or Pyr:GFP under pyr-Gal4 (B, B’). C, D’ Images of wing discs harboring mCherryCAAX-marked AMPs; wing discs expressing either Ths:GFP or Pyr:GFP as indicated. A–D’ dashed arrow, signal in the source; arrow, non-autonomous punctate distribution in the AMP; *, non-expressing areas of the lacking signal distribution. E, F’ Wing discs expressing either Ths:GFP or Pyr:GFP as indicated, showing niche-specific effects of signal overexpression on proliferation of niche-resident and non-resident AMPs (nls:GFP marked); thick and thin dashed line, interface between AMPs and ths-expression and pyr-expression zones, respectively; dashed and solid arrows, effects on ths-expression and pyr-expression zones, respectively. G–J YZ views of wing discs expressing either Ths:GFP or Pyr:GFP as indicated and harboring mCherryCAAX-marked AMPs, showing disc-invading cytonemes receiving GFP-tagged signal (arrow) from the disc cells; arrowheads, localized signal enrichment in source cells. Scale bars: 20μm; 5 μm (H, H’).