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. 2022 Mar 4;13:1169. doi: 10.1038/s41467-022-28785-9

Fig. 7. Wnt /β-catenin signaling regulation by Unc5B-blocking antibodies.

Fig. 7

a Immunofluorescence staining of Human IgG, Claudin-5 and PLVAP and confocal imaging on brain sections from mice i.v. injected with anti-Unc5B-3 (10 mg/kg) for 1 h, 8 h or 24 h, reproduced on n = 3 untreated brains, n = 4 anti-Unc5B-3 treated brains for 1 h, n = 3 anti-Unc5B-3 treated brains for 8 and n = 3 anti-Unc5B-3 treated brains for 24 h. b CTRL IgG or Unc5B immunoprecipitation of brain protein extracts from mice i.v. injected with anti-Unc5B-3 and protein quantification (c), n = 3 control IgG treated brains, n = 4 anti-Unc5B-3 treated brains for 1 h and n = 3 anti-Unc5B-3 treated brains for 8 and n = 3 anti-Unc5B-3 treated brains for 24 h. Each dot represents one mouse. One control mouse was set as 1. All data are shown as mean ± SEM. NS non-significant. ANOVA followed by Bonferroni’s multiple comparisons test was performed for statistical analysis between multiple groups. Source data are provided as a Source Data file.