mRNA expression of Ern1 (A), Itpr3 (B), Eif2ak3 (C), Eif4g1 (D) and protein expression of IRE1 (E) and PERK (F) of mouse placental samples collected at E12.5, E14.5, E16.5 and E18.5 (from three to six litters per gestational age). Significance was indicated when P< 0.05. Significant differences between groups as detected by post hoc analysis are denoted by different letters where A is different from B. A is not different from A or AB, while B is not different from B or AB. Data are presented as mean ± s.e.m. (n = 10, five per sex for gene analysis and n = 6, three per sex for protein analysis).