Fig. 8.

Felodipine in combination with gentamicin alleviates murine periprosthetic joint infection. (A) Schematic diagram of the treatment of implant infections in mice. (B) After treatment with felodipine (40 mg/kg, s.c.), gentamicin (80 mg/kg, s.c.), or the combination of felodipine (40 mg/kg, s.c.) and gentamicin (80 mg/kg, s.c.), the infected joint was examined using X-ray and Micro-CT to evaluate periosteal reaction, osteolysis and the position of Ti6Al4V rod. (C) Radiographic scores of infected joints. The evidence for scoring includes five aspects. Each aspect was scored on a five-point scale (0–4), where 4 represented the most severe. (D) Bone mineral density and (E) bone volume fraction (BV/TV) were measured using Micro-CT. Data are expressed as the means ± SD; n = 10; ns, not significant; **p < 0.01, ***p < 0.001. (F) Bacterial morphology and biofilm viability on the surface of Ti6Al4V rod were detected by SEM and CLSM. (G) Distribution of CFU density per implant was determined by spread plate method. Data are expressed as the means ± SD; n = 10; *p < 0.05; ***p < 0.001. (H) Distribution of CFU density per soft tissue was determined by spread plate method. Data are expressed as the means ± SD; n = 10; ns, not significant; **p < 0.01, ***p < 0.001.