Table 1.
List of culture formulations and expansion conditions
| Condition | Abbreviation | Stimulation | Medium | Glucose (mM) | Glutamine (mM) |
|---|---|---|---|---|---|
| Rapid Expansion Protocol | REP | Soluble αCD3 and 50 Gy-irradiated PBMCs | AIM-V Medium and RPMI1640 (CTL:AIM-V) 5% human serum |
12.40 (SD ± 0.40) | 3.75 (SD ± 0.12) |
| StemCell | ICM | ImmunoCult Human CD3/CD28 T cell Activators | ImmunoCult-XF T cell Expansion Medium no human serum |
22 (SD ± 2.21) | 6.68 (SD ± 0.29) |
| Miltenyi | TAC | T cell CD3/CD28 TransAct | TextMACS Medium 3% human serum |
17 (SD ± 0.89) | 2.19 (SD ± 0.04) |
| Corning | CMM | plate-bound αCD3 and soluble αCD28 | Corning 88-581-CM Medium 3% human serum |
27.25 (SD ± 0.63) | 3.68 (SD ± 0.11) |
| Human Plasma-like Medium | HPLM | plate-bound αCD3 and soluble αCD28 | Human Plasma-like Medium 3% human serum |
5.92 (SD ± 0.33) | 0.76 (SD ± 0.04) |
Glucose and glutamine concentrations were measured using colorimetric assay.