. 2022 Mar 5;13:34. doi: 10.1186/s40104-022-00683-6

Table 1.

Effect of different doses of Lactobacillus reuteri, and a mixture of reuterin and microcin J25 on growth performance of broiler chickens from d 0 to 35 of experiment

Variables	Treatments
Variables	PC	NC	1RJ	5RJ	10RJ	E6Lr	E7Lr	E8Lr	SEM	P-value
*Feed intake, kg*
d 0 to d 10	0.29^a	0.27^bc	0.28^ab	0.28^ab	0.29^a	0.26^cd	0.26^d	0.26^cd	0.004	<.0001
d 11 to d 22	1.18^a	1.09^c	1.12^abc	1.13^abc	1.15^ab	1.12^bc	1.09^c	1.09^c	0.013	<.0001
d 0 to d 22	1.47^a	1.36^cd	1.40^bcd	1.41^abc	1.44^ab	1.38^bcd	1.34^d	1.35^cd	0.015	<.0001
d 23 to d 35	2.41^a	2.28^b	2.37^ab	2.32^ab	2.40^a	2.33^ab	2.28^b	2.35^ab	0.027	0.001
d 0 to d 35	3.87^a	3.64^c	3.77^abc	3.73^bc	3.83^ab	3.71^bc	3.63^c	3.70^bc	0.037	<.0001
*Body weight, kg*
d 0	0.05	0.05	0.05	0.05	0.05	0.05	0.05	0.05	0.0002	0.350
d 7	0.20^a	0.19^abc	0.20^ab	0.19^abc	0.20^a	0.18^c	0.19^bc	0.19^c	0.004	<.0001
d 10	0.31^a	0.30^c	0.30^bc	0.30^bc	0.31^ab	0.30^bc	0.30^c	0.30^abc	0.004	0.0002
d 22	1.19^a	1.12^c	1.13^bc	1.15^abc	1.17^ab	1.13^c	1.12^c	1.13^c	0.017	<.0001
d 35	2.82^a	2.65^c	2.73^abc	2.68^bc	2.77^ab	2.70^bc	2.67^bc	2.70^bc	0.026	0.0001
*Average daily gain, g*
d 0 to d 7	22.3^a	21.0^bcd	21.4^abc	21.3^abcd	22.2^ab	19.5^d	20.2^cd	20.2^d	0.53	<.0001
d 8 to d 10	37.8^abc	34.5^c	35.4^bc	36.0^bc	37.2^abc	39.9^a	36.8^abc	38.7^ab	0.84	0.0001
d 0 to d 10	27.0^a	25.1^c	25.6^bc	25.7^abc	26.7^ab	25.6^abc	25.2^cd	25.7^abc	0.35	0.0001
d 11 to d 22	72.7^a	68.9^b	68.9^b	70.7^ab	71.4^ab	68.9^b	68.4^b	68.5^b	0.97	0.001
d 0 to d 22	51.9^a	49.0^c	49.3^bc	50.3^abc	51.1^ab	49.2^bc	48.8^c	49.1^bc	0.63	<.0001
d 23 to d 35	125.2^a	117.6^b	123.0^ab	117.3^b	123.3^ab	120.9^ab	119.3^ab	121.0^ab	1.47	0.003
d 0 to d 35	79.1^a	74.5^c	76.7^abc	75.2^bc	77.9^ab	75.9^bc	75.0^bc	75.8^bc	0.74	0.0001
*Feed conversion ratio, g:g*
d 0 to d 10	1.10^b	1.12^b	1.12^b	1.12^b	1.10^b	1.04^a	1.06^a	1.05^a	0.006	<.0001
d 11 to d 22	1.50^a	1.54^b	1.52^ab	1.51^ab	1.51^ab	1.49^a	1.49^a	1.51^ab	0.010	0.012
d 0 to d 22	1.39^abc	1.42^d	1.41^cd	1.40^bcd	1.40^abcd	1.37^a	1.38^ab	1.39^abc	0.007	<.0001
d 23 to d 35	1.50	1.50	1.51	1.53	1.52	1.50	1.50	1.50	0.013	0.088
d 0 to d 35	1.45^abc	1.47^abc	1.47^abc	1.47^c	1.47^bc	1.45^ab	1.45^a	1.45^abc	0.005	0.001
*Mortality, %*
d 0 to d 7	5.5	6.6	6.6	4.0	4.4	4.8	7.7	7.7	1.6	0.425
d 8 to d 10	1.0	2.0	2.0	2.3	1.3	1.0	1.6	1.3	1.0	0.856
d 11 to d 22	3.7^ab	9.2^b	4.0^ab	6.6^ab	5.2^ab	2.6^a	5.2^ab	5.9^ab	1.8	0.038
d 23 to d 35	2.4	1.7	0.7	1.0	2.0	1.7	1.4	1.4	1.0	0.789
d 0 to d 35	12.9^ab	19.9^b	13.6^ab	14.3^ab	13.2^ab	10.3^a	16.2^ab	16.5^ab	2.4	0.045

^a,b,c,d means in each raw with different superscripts are significantly different (P < 0.05). SEM = standard error of means

PC = antibiotic bacitracin, NC = without antibiotic, 1RJ = 0.5 mmol/L reuterin and 0.008 μmol/L J25, 5RJ = 2.5 mmol/L reuterin and 0.04 μmol/L J25, 10RJ = 5 mmol/L reuterin and 0.08 μmol/L J25, E6Lr = L. reuteri (10⁶ CFU/mL) plus 300 mmol/L glycerol, E7Lr = L. reuteri (10⁷ CFU/mL) plus 300 mmol/L glycerol, E8Lr = L. reuteri (10⁸ CFU/mL) plus 300 mmol/L glycerol. (n = 8 replicates/treatment)