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. 2022 Mar 5;18:86. doi: 10.1186/s12917-022-03186-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — Acrosome integrity and capacitation status of the canine post-thaw sperm evaluated by CTC staining after cryopreservation in extenders containing different levels of melatonin versus the control groups. One-way ANOVA statistical test was used to make a comparison between treatment groups and the LSD posthoc test was used to perform pairwise comparisons. Different letters in bar superscripts indicate a statistically significant difference (P ≤ 0.05). Negative Control (without Melatonin or DMSO), Positive Control (containing DMSO), M1 (containing 0.5 mM Melatonin), M2 (containing 1 mM Melatonin), and M3 (containing 2 mM Melatonin). All numbers are presented as Means±SEM. Pattern F = non-capacitated with intact acrosome, pattern B = capacitated with intact acrosome, and Pattern AR = reacted/damaged acrosome, CTC=Chlortetracycline