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. 2021 Dec 15;26(3):2949–2963. doi: 10.1007/s00784-021-04277-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — CAP adhesion assay. A–D Adjusted percentage of cell adhesion after (24 h/48 h) of different CAP treatment modalities. Untreated specimens/cells served as control, surface treatment (ST), cell treatment (CT); columns and error bars represent the mean ± SEM (n = 3). E/H Cell quantification using ImageJ Software, microscopic detection of cell colony within the area of interest (AI) (× 50). F/I Image transformation for cell detection using 8-bit resolution (Process: Binary: Make Binary) with cell separation G/J automatic cell counting (Menu: Analyse Particles) narrowing particle size (Size: 25-Infinity/Circularity: 0.00–1.00)