Figure 2.

Patients with tumors that are larger in diameter, higher grade, left laterality, and sarcomatoid status have increased diversity in TRA and IGL receptors. A, TRA receptor CDR3 sequence ΔD diversity across the Moffitt TCC cohort have increased diversity in (i) larger diameter tumors (low diversity had mean diameter of 6.1 cm and high diversity had a mean diameter for 7.6 cm; P: 0.0287), (ii) left laterality tumors (score of 37.55 vs. 16.39; P: 0.0097), (iii) with higher grade tumors (mean score from grade 2 was 12.96, mean score from grade 3 was 33.09, and mean score from grade 4 was 42.62; P: 0.0227), and (v) in patients with sarcomatoid status [sarcomatoid status evaluated as yes (at least 5%) or no, in TRA no had a mean ΔD diversity score of 26.47 vs. yes with a mean score of 61.32; P 0.0430]. B, IGL receptor CDR3 sequence ΔD diversity showed the same trends as TRA receptor CDR3 sequence diversity for (i) size (low diversity had mean diameter of 6.1 cm and high diversity had a mean diameter of 7.6 cm: P: 0.0195), (ii) laterality (score of 331.1 vs. 141.3; P: 0.0445), (iii) grade (mean score from grade 2 was 93.99, mean score from grade 3 was 281.4, and mean score from grade 4 was 465.5; P: 0.0459), and (iv) sarcomatoid status (no had a mean score of 223.9 vs. yes with a mean score of 704.4; P: 0.0152). Unpaired t tests were used to compare two group data and ANOVA was used to compare grade, three group data. *, P < 0.05; **, P < 0.01.