Figure 1: Fiber Fluorescence Intensity and Digestion Rates.
A) Plots showing the average cross-sectional fluorescence intensity as a function of time for representative locations. Locations of 9 μm and 19 μm are shown, which correspond to the locations marked by the orange lines in panel B. Red lines show linear fits to the data which allow a quantification of the average rate of change of the fluorescence intensity, and hence the average rate of change of fiber diameter. B) Snapshots of the fibrin fiber showing the fluorescence intensity along the fiber at different points in time. C) The average rate of change of fluorescence intensity at every location along the fiber. Rate of digestion was calculated at each location as the slope of the fluorescence vs time plot at that location (red lines in panel A).