Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 5;8:99. doi: 10.1038/s41420-022-00902-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — A, B The results of the dot blot assay showed that NSUN5 knockdown reduced 5mC levels, whereas NSUN5 overexpression elicited the opposite effect. *p < 0.05. C Compared with control cells, 5mC levels were reduced in NSUN5-depleted cells, as determined by immunofluorescence analysis. *p < 0.05. D 5mC antibody enriched FTH1 and FTL mRNA, whereas NSUN5 knockdown reduced 5mC levels in FTH1 and FTL mRNA, as determined by RNA immunoprecipitation (RIP) and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. *p < 0.05. E Methylation sites of FTH1 and FTL mRNA in control of NSUN5-depleted BMSCs were mainly in the 5′ or 3′ UTR. F, G Bisulfite-sequencing analysis revealed that NSUN5 knockdown abolished the C-to-T conversion at FTH1 and FTL mRNA cytosine methylation sites, *p < 0.05 (D).