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. 2022 Mar 5;8:101. doi: 10.1038/s41420-022-00899-5

Fig. 1. The FAS receptor regulates in vitro OSCC stemness and progression and in vivo lung colony formation.

Fig. 1

A Western blot analysis of FAS and β-actin protein expression in control and FAS−/− SAS cells. B OCT4 reporter activity between control and FAS−/− SAS cells. C CSC sphere formation assay between control and FAS−/− SAS cells. Scale bar: 100 μm. D CDH1 reporter activity between control and FAS−/− SAS cells. E, F Cellular migration (E) and invasion (F) ability of SAS FAS−/− cells. Scale bar: 100 μm. G IVIS images of SAS-Luc control and FAS−/−-Luc cells (n = 5/group). H Lung H&E staining of SAS-Luc control and FAS−/− cells. H&E staining reveals high pulmonary hemorrhage and diffuse cancer growth. Scale bar: 300 μm. I H&E staining of parental SAS lung colony formation assay with or without FAS neutralizing antibody (FAS-Neut-Ab) pretreatment. Scale bar: 300 μm. J The mouse survival curve of parental SAS cells with or without FAS-Neut-Ab pretreatment.