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. 2022 Mar 5;8:101. doi: 10.1038/s41420-022-00899-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Global mRNA expression in FAS^−/− SAS cells analyzed by an Affymetrix U133 cDNA microarray. The significantly differentially expressed genes (fold change = 2) were further analyzed by IPA upstream regulator in z-score to reveal the differential signaling pathways in FAS^−/− cells. B Promoter reporter assays of cancer-related signaling pathways between control and FAS^−/− SAS cells. C JAG1 mRNA expression level in control and FAS^−/− SAS cells. D JAG1 mRNA expression in OSCC patients E Pearson correlation analysis of FAS and JAG1 mRNA expression in OSCC patients. F The CSC sphere formation assay in FAS^−/− SAS cells with or without restoration of the NOTCH1-ICD. Scale bar: 100 μm.