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. 2021 Aug 27;17(14):3745–3759. doi: 10.7150/ijbs.57610

Figure 2.

Figure 2

Increased miR-9-5p strongly correlates to inflammatory cell infiltration in liver tissues of HFD-fed mice. A, The expression of TG and TC in mice plasma detected by corresponding assay kits. B, The expression of AST and ALT in mouse plasma samples detected by biochemical analyzer. C, miR-9-5p level in liver tissues of HFD-fed mice measured by RT-qPCR. D, miR-9-5p level in plasma-derived EVs measured by RT-qPCR. E, The number of M1 macrophages F4/80+ CD86+ in mouse liver tissues detected by immunofluorescence staining. F, Levels of M1 macrophage surface markers in liver tissues of HFD-fed mice determined by RT-qPCR. n = 6. * p < 0.05 vs. normal mice. Data are shown as the mean ± standard deviation of three independent experiments. Unpaired t-test was used for analysis of differences between two groups.