Skip to main content
. 2022 Feb 7;18(4):1663–1676. doi: 10.7150/ijbs.69405

Figure 5.

Figure 5

MiR-186-5p was responsible for the promotion effects of M2-EVs. (A) RT-PCR was used to detect the expression level of miR-186-5p in CC cells stimulated by M2-EVs (*p<0.05, **p<0.01). (B-C) Images of SW480 (scale bar = 20μm) and HCT-8 (scale bar = 10μm) were taken after cocultured with M2 macrophages transfected with Cy3-labeled miR-186-5p mimics (Cy3-mimics). (D) RT-PCR was used to detect the expression of miR-186-5p in M2 macrophage and M2-EVs after transfected with its inhibitor (***p<0.001). (E-G) MTS and EdU assay was used to detect the proliferation rates of CC cells stimulated by M2-EVs with reduced miR-186-5p level (scale bar = 100μm) (**p<0.01, ***p<0.001). (H-K) Transwell migration and invasion assay was used to determine the motility of CC cells stimulated by M2-EVs with reduced miR-186-5p level. (scale bar = 100μm) (**p<0.01, ***p<0.001, ****p<0.0001).