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. 2022 Jan 24;18(4):1415–1433. doi: 10.7150/ijbs.69454

Figure 7.

Figure 7

lncRNA BDNF-AS could promote gastric cancer tumorigenesis and peritoneal metastasis in vivo . Representative images of migratory or invaded cells were shown. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data were shown as mean ± SEM (n = 3). (A): The morphological properties of tumor subcutaneous xenograft, tumor size and tumor weight in PO-BDNF-AS-HGC-27 cells, PONC-BDNF-AS-HGC-27 cells, and PO-BDNF-AS+PO-FBXW7-HGC-27 cells at 30 days, each group had five mice. (B): IHC analyzed the expression of FBXW7, WDR5, and VDAC3 proteins of tumors from the PO-BDNF-AS-HGC-27 cells, PONC-BDNF-AS-HGC-27 cells, and PO-BDNF-AS+PO-FBXW7-HGC-27 cells groups. (C-D): The image of intraperitoneal tumor formation model from PO-BDNF-AS-HGC-27 and PONC-BDNF-AS-HGC-27 cells at five weeks (C). Simultaneously we counted and analyzed the number of the metastases in peritoneal, perigastric, mesenteric and diaphragmatic (D), each group had six mice. (E): The mRNA relative expression levels of FBXW7, WDR5, and VDAC3 of tumors were detected by qRT-PCR assay from the PO-BDNF-AS-HGC-27 cells, PONC-BDNF-AS-HGC-27 cells, and PO-BDNF-AS+PO-FBXW7-HGC-27 cells groups. (F): The protein expression level of VDAC3 was analyzed by western blotting from the PO-BDNF-AS-HGC-27 cells, PONC-BDNF-AS-HGC-27 cells, and PO-BDNF-AS+PO-FBXW7-HGC-27 cells groups. (G): Proposed the mechanism model in which BDNF-AS/WDR5/FBXW7 axis mediates ferroptosis in GC PM by regulating VDAC3 protein.