Figure 1.

NRF2 overexpression impairs the H/R induced mitochondrial ROS generation in adult cardiomyocytes. (A) The cellular viability was detected by CCK-8 assay; (B) The percentage of apoptotic cardiomyocytes was analyzed by TUNE staining; (C) The level of cTnI in the culture medium were also detected at the end of the reperfusion to assess myocardial injury; (D) The activity of LDH and (E) CK were evaluated by Electrochemiluminescence immunoassay; (F) The total cellular and (G) mitochondrial ROS were analyzed by DCFH-DA and Mito-SOX assay; (H) The levels of SOD and (I) GSH were analyzed by ELISA Kit. N=3; *P<0.05, **P<0.01 verses indicated group.