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. 2022 Feb 7;18(4):1651–1662. doi: 10.7150/ijbs.63297

Figure 4.

Figure 4

Notch1 impairs the Keap1-Cullin3 interaction in adult cardiomyocytes. (A) The protein level of Notch1, Keap1 and Cullin3 were confirmed by Western Blot; (B) Co-immunoprecipitation assay was used to analysis the interaction between Notch1, Keap1 and Cullin3. (C) Immunofluorescence observation of the distribution of Keap1 and Cullin3 by confocal microscopy. N=3.