Characterization of MsEVs from different cell types. (A-E) The origin of BALF MsEVs classified by flow cytometry. CD11c+ and F4/80+ events designate alveolar macrophages; EpCAM+—epithelial cells; other events (such as CD31+ for endothelial cells, CD11c+ F4/80- for dendritic cells, CD11c- F4/80+ for interstitial macrophages) were pooled into the Other group. (F and G) Quantitative measurement of MsEVs. MsEVs from different cell sources were detected at different time points in the early phase of the ALI model (n=6). Data are expressed as the mean ± SD. AM, alveolar macrophage; IM, interstitial macrophage; DC, dendritic cell; EpC, epithelial cell; NC, neutrophil; EC, endothelial cell.